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It has been suggested that bacterial infection induces DNA synthesis in the nuclei of urothelial cells in the urinary tract. An indicator of cellular DNA synthesis would be an increased utilization of thymidine, an analogue of deoxycytidine, a deoxynucleotide commonly used in DNA synthesis. Urothelial cells were incubated with [3H]thymidine in the presence or absence of antibiotics and the distribution of thymidine between the water-soluble and insoluble fractions was determined. The distribution of the label between the water-soluble and insoluble fractions was similar in the control and antibiotic-treated groups. An increase in the total radioactivity of the insoluble fractions was observed in the antibiotic-treated cells. These results suggest that the antibiotic treatment increased the rate of thymidine utilization in DNA synthesis in urothelial cells.Dynamics of macrophage polarization after renal ischemia-reperfusion injury.
Acute renal ischemia-reperfusion
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