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Category:Latin American musicThe following description of the background of the invention is provided to aid in understanding the invention, but is not admitted to be or describe prior art to the invention.
Human Papilloma Virus (HPV) infections have been linked to several types of anogenital cancers. The risk of developing HPV-associated cancers is directly related to the level of viral DNA detected in the malignant cells. The viral oncogenes E6 and E7 are frequently expressed in HPV-associated cancers, suggesting that the HPV genes may play a role in the development of cancer.
The E6 and E7 genes from high risk types of HPV have been cloned and expressed in E. coli, yeast, insect cells and in animal cell culture. The E6 and E7 proteins have been shown to form stable heterodimers in vitro and in yeast and insect cells (Zhong et al., 2001).
RAS, a Ras GTPase superfamily member, regulates growth and differentiation in a wide variety of organisms. Activating mutations of RAS genes have been found in human cancers, including 90% of all pancreatic cancers, 40% of all breast cancers, 25-30% of lung cancers, and 15% of colorectal cancers. RAS mutations occur most frequently at codon 12 of the HRas gene and a single point mutation in HRas at residue 61 (Gly-to-Asp) occurs in approximately 50% of all pancreatic cancers.
RAS genes are critical to the physiological functions of animals and humans. Mutations of RAS genes occur at a frequency of 6.5% in humans. Activating RAS mutations occur in 21% of all human cancers, 18% of lung cancers, 14% of breast cancers, and 27% of colorectal cancers, suggesting that Ras is one of the most frequently mutated genes in human cancer. Only 1% of all human cancers have mutations in the p53 gene, but 10% of all human tumors show mutation in RAS genes (Harris et al., 2001).
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