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A practical method for the selective recovery of Escherichia coli endotoxin from aqueous and alcoholic media is described. This method allows the LAL test to be used in a variety of samples, including milk, culture media, all lots of sterile water, and synthetic extracellular products of E. coli K12. The method consists of a simple filtration of the sample through 0.22-micron filter, followed by hemolysis and subsequent mixing with amoebocytes. With this procedure, endotoxin of E. coli that was present in sample below an endotoxin assay limit of detection can be measured by the LAL test. Similarly, lactate dehydrogenase (LDH) in the samples can be measured simultaneously to indicate the amount of sample lysed by the method. The same procedure was adapted for the isolation of E. coli endotoxin from alcoholic media and for the recovery of lecithin and cholesterin from egg yolk, and the amount of each isolated compound was measured by standard enzyme techniques. The described method offers a means of highly selective extraction of the desired molecule from a complex mixture and allows for rapid and simple determination of the amount of endotoxin present.- As reported earlier, The Porter’s X Factor confirmed this week that they will be replacing judge Phillip Phillips on the new season of the show. News of their replacements comes following Phillips’ departure from the show on September 10th.
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